› 4x1t

X-ray diffraction
2.25Å resolution

The crystal structure of Arabidopsis thaliana galactolipid synthase MGD1 in complex with UDP

Released:
DOI: 10.2210/pdb4x1t/pdb
PDB entry 4x1t coloured by chain, front view.
PDB entry 4x1t coloured by chain, side view.
PDB entry 4x1t coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights and membrane binding properties of MGD1, the major galactolipid synthase in plants.
Rocha J, Sarkis J, Thomas A, Pitou L, Radzimanowski J, Audry M, Chazalet V, de Sanctis D, Palcic MM, Block MA, Girard-Egrot A, Maréchal E, Breton C
Plant J 85 622-33 (2016)
PMID: 26935252

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monogalactosyldiacylglycerol synthase 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 45.33 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O81770 (Residues: 137-533; Coverage: 75%)
Gene names: At4g31780, EMB2797, F28M20.30, MGD1, MGDA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand UDP 1 x UDP
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P63
Unit cell:
a: 139.25Å b: 139.25Å c: 38.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Role of membrane glycerolipids in photosynthesis, thylakoid biogenesis and chloroplast development.
Kobayashi K. (2016)
4 more

2 mentions without citation

Monogalactosyldiacylglycerol and Sulfolipid Synthesis in Microalgae.
Riccio et al. (2020)
1 more