› 4x28

X-ray diffraction
1.99Å resolution

Crystal structure of the ChsE4-ChsE5 complex from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4x28/pdb
PDB entry 4x28 coloured by chain, front view.
PDB entry 4x28 coloured by chain, side view.
PDB entry 4x28 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Unraveling Cholesterol Catabolism in Mycobacterium tuberculosis: ChsE4-ChsE5 α2β2 Acyl-CoA Dehydrogenase Initiates β-Oxidation of 3-Oxo-cholest-4-en-26-oyl CoA.
Yang M, Lu R, Guja KE, Wipperman MF, St Clair JR, Bonds AC, Garcia-Diaz M, Sampson NS
ACS Infect Dis 1 110-125 (2015)
PMID: 26161441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125197 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyl-CoA dehydrogenase FadE26 Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.3 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: I6YCA3 (Residues: 1-400; Coverage: 100%)
Gene names: Rv3504, fadE26
Sequence domains:
Structure domains:
Acyl-CoA dehydrogenase FadE27 Chains: C, D
Molecule details ›
Chains: C, D
Length: 373 amino acids
Theoretical weight: 39.19 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: I6Y3Q0 (Residues: 1-373; Coverage: 100%)
Gene names: Rv3505, fadE27
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FDA 2 x FDA
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 185.745Å b: 108.095Å c: 82.019Å
α: 90° β: 93.04° γ: 90°
R-values:
R R work R free
0.153 0.151 0.186
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Cholesterol metabolism: a potential therapeutic target in Mycobacteria.
Abuhammad A. (2017)
6 more

3 mentions without citation

Unraveling Cholesterol Catabolism in Mycobacterium tuberculosis: ChsE4-ChsE5 α2β2 Acyl-CoA Dehydrogenase Initiates β-Oxidation of 3-Oxo-cholest-4-en-26-oyl CoA.
Yang et al. (2015)
2 more