› 4x3l

X-ray diffraction
1.7Å resolution

Crystal Structure of TTHA0275 from Thermus thermophilus (HB8) in complex with 5'-methylthioadenosine in space group P21212

Released:
DOI: 10.2210/pdb4x3l/pdb
PDB entry 4x3l coloured by chain, front view.
PDB entry 4x3l coloured by chain, side view.
PDB entry 4x3l coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Demirci H, Belardinelli R, Jogl G

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA 2-O ribose methyltransferase substrate binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 28.04 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLL8 (Residues: 1-260; Coverage: 100%)
Gene name: TTHA0275
Sequence domains: SpoU rRNA Methylase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MTA 2 x MTA
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 111.17Å b: 115.95Å c: 58.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.207
Expression system: Escherichia coli BL21(DE3)

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