› 4x4z

X-ray diffraction
1.8Å resolution

Retrofitting antibodies with stabilizing mutations. Herceptin VL mutant F53D.

Released:
DOI: 10.2210/pdb4x4z/pdb
PDB entry 4x4z coloured by chain, front view.
PDB entry 4x4z coloured by chain, side view.
PDB entry 4x4z coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Langley DB, Rouet R, Christ D

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Herceptin VL domain with F53D mutation Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: F222
Unit cell:
a: 98.58Å b: 103.75Å c: 110.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.213
Expression system: Escherichia coli BL21(DE3)

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