› 4x5n

X-ray diffraction
3Å resolution

Crystal structure of SemiSWEET in the inward-open and outward-open conformations

Released:
DOI: 10.2210/pdb4x5n/pdb
PDB entry 4x5n coloured by chain, front view.
PDB entry 4x5n coloured by chain, side view.
PDB entry 4x5n coloured by chain, top view.
Source organism: Escherichia coli UMEA 3162-1
Primary publication:
Structural basis for the facilitative diffusion mechanism by SemiSWEET transporter.
Lee Y, Nishizawa T, Yamashita K, Ishitani R, Nureki O
Nat Commun 6 6112 (2015)
PMID: 25598322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143645 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar transporter SemiSWEET Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 100 amino acids
Theoretical weight: 11.23 KDa
Source organism: Escherichia coli UMEA 3162-1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV3 (Residues: 1-89; Coverage: 100%)
Gene name: G925_04926
Sequence domains: PQ loop repeat
Structure domains: Monooxygenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 118.01Å b: 34.63Å c: 123.22Å
α: 90° β: 102.93° γ: 90°
R-values:
R R work R free
0.285 0.281 0.328
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
11 more

5 mentions without citation

GLUT, SGLT, and SWEET: Structural and mechanistic investigations of the glucose transporters.
Deng et al. (2016)
4 more