› 4x8f

X-ray diffraction
3.4Å resolution

Vibrio cholerae O395 Ribokinase in apo form

Released:
DOI: 10.2210/pdb4x8f/pdb
Model geometry
Fit model/data
PDB entry 4x8f coloured by chain, front view.
PDB entry 4x8f coloured by chain, side view.
PDB entry 4x8f coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
Crystal structure of apo and ligand bound vibrio cholerae ribokinase (Vc-RK): role of monovalent cation induced activation and structural flexibility in sugar phosphorylation.
Paul R, Patra MD, Sen U
Adv Exp Med Biol 842 293-307 (2015)
PMID: 25408351

Function and Biology Details

Reaction catalysed: 
ATP + D-ribose = ADP + D-ribose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribokinase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 309 amino acids
Theoretical weight: 32.39 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UL04 (Residues: 4-309; Coverage: 99%)
Gene names: VC395_A0124, rbsK
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 129.22Å b: 130.849Å c: 145.69Å
α: 110.52° β: 90° γ: 119.59°
R-values:
R R work R free
0.242 0.239 0.308
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Molecular Mechanisms of Enzyme Activation by Monovalent Cations.
Gohara et al. (2016)
7 other articles