› 4x8h

X-ray diffraction
2.5Å resolution

Crystal structure of E. coli Adenylate kinase P177A mutant

Released:
DOI: 10.2210/pdb4x8h/pdb
PDB entry 4x8h coloured by chain, front view.
PDB entry 4x8h coloured by chain, side view.
PDB entry 4x8h coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for catalytically restrictive dynamics of a high-energy enzyme state.
Kovermann M, Ådén J, Grundström C, Sauer-Eriksson AE, Sauer UH, Wolf-Watz M
Nat Commun 6 7644 (2015)
PMID: 26138143

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69441 (Residues: 1-214; Coverage: 100%)
Gene names: JW0463, adk, b0474, dnaW, plsA
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR-H
Spacegroup: C2
Unit cell:
a: 135.418Å b: 31.534Å c: 52.745Å
α: 90° β: 110.65° γ: 90°
R-values:
R R work R free
0.196 0.191 0.29
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein dynamics and function from solution state NMR spectroscopy.
Kovermann et al. (2016)
21 other articles

1 mention without citation

Structural basis for catalytically restrictive dynamics of a high-energy enzyme state.
Kovermann et al. (2015)