› 4xa4

X-ray diffraction
2.33Å resolution

Crystal Structure of the coiled-coil surrounding Skip 3 of MYH7

Released:
DOI: 10.2210/pdb4xa4/pdb
PDB entry 4xa4 coloured by chain, front view.
PDB entry 4xa4 coloured by chain, side view.
PDB entry 4xa4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly.
Taylor KC, Buvoli M, Korkmaz EN, Buvoli A, Zheng Y, Heinze NT, Cui Q, Leinwand LA, Rayment I
Proc Natl Acad Sci U S A 112 E3806-15 (2015)
PMID: 26150528

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein XRCC4; Myosin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13426 (Residues: 2-147; Coverage: 44%)
  • Canonical: P12883 (Residues: 1551-1609; Coverage: 3%)
Gene names: MYH7, MYHCB, XRCC4
Sequence domains: DNA double-strand break repair and V(D)J recombination protein XRCC4
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 54.548Å b: 90.195Å c: 102.717Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Disease mutations in striated muscle myosins.
Parker et al. (2020)
1 more

7 mentions without citation

In-Cell Labeling and Mass Spectrometry for Systems-Level Structural Biology.
Chavez et al. (2022)
6 more