› 4xbh

X-ray diffraction
2.11Å resolution

Soluble rabbit neprilysin

Released:
DOI: 10.2210/pdb4xbh/pdb
PDB entry 4xbh coloured by chain, front view.
PDB entry 4xbh coloured by chain, side view.
PDB entry 4xbh coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan.
Labiuk SL, Sygusch J, Grochulski P
Acta Crystallogr F Struct Biol Commun 75 405-411 (2019)
PMID: 31204686

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-139899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neprilysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 696 amino acids
Theoretical weight: 79.48 KDa
Source organism: Oryctolagus cuniculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08049 (Residues: 55-750; Coverage: 93%)
Gene name: MME
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ZN 2 x ZN
Ligand PO4 2 x PO4
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 69.361Å b: 108.142Å c: 212.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.211
Expression system: Komagataella pastoris

Quick links

Citations

1 citations in other articles

Molecular Basis for Omapatrilat and Sampatrilat Binding to Neprilysin-Implications for Dual Inhibitor Design with Angiotensin-Converting Enzyme.
Sharma et al. (2020)

3 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
2 more