› 4xdj

X-ray diffraction
3.8Å resolution

Crystal structure of human two pore domain potassium ion channel TREK2 (K2P10.1) in an alternate conformation (FORM 2)

Released:
DOI: 10.2210/pdb4xdj/pdb
PDB entry 4xdj coloured by chain, front view.
PDB entry 4xdj coloured by chain, side view.
PDB entry 4xdj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac.
Dong YY, Pike AC, Mackenzie A, McClenaghan C, Aryal P, Dong L, Quigley A, Grieben M, Goubin S, Mukhopadhyay S, Ruda GF, Clausen MV, Cao L, Brennan PE, Burgess-Brown NA, Sansom MS, Tucker SJ, Carpenter EP
Science 347 1256-9 (2015)
PMID: 25766236

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel subfamily K member 10 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 282 amino acids
Theoretical weight: 31.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P57789 (Residues: 62-335; Coverage: 51%)
Gene names: KCNK10, TREK2
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand K 6 x K
Ligand PC1 4 x PC1
Ligand TRD 4 x TRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 76.745Å b: 113.871Å c: 111.782Å
α: 90° β: 90.97° γ: 90°
R-values:
R R work R free
0.264 0.263 0.277
Expression system: Spodoptera frugiperda

Quick links

Citations

30 review citations

Mechanically Activated Ion Channels.
Ranade et al. (2015)
29 more

20 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
19 more