› 4xey

X-ray diffraction
2.89Å resolution

Crystal structure of an SH2-kinase domain construct of c-Abl tyrosine kinase

Released:
DOI: 10.2210/pdb4xey/pdb
PDB entry 4xey coloured by chain, front view.
PDB entry 4xey coloured by chain, side view.
PDB entry 4xey coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an SH2-kinase construct of c-Abl and effect of the SH2 domain on kinase activity.
Lorenz S, Deng P, Hantschel O, Superti-Furga G, Kuriyan J
Biochem J 468 283-91 (2015)
PMID: 25779001

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ABL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 46.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00519 (Residues: 119-515; Coverage: 35%)
Gene names: ABL, ABL1, JTK7
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 1N1 2 x 1N1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 114.118Å b: 125.449Å c: 56.232Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Multifunctional Abl kinases in health and disease.
Khatri et al. (2016)
1 more

7 mentions without citation

The Src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases.
Shah et al. (2018)
6 more