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› 4xf4

X-ray diffraction
1.35Å resolution

Cysteine dioxygenase at pH 8.0 in complex with homocysteine

Released:
DOI: 10.2210/pdb4xf4/pdb
Model geometry
Fit model/data
PDB entry 4xf4 coloured by chain, front view.
PDB entry 4xf4 coloured by chain, side view.
PDB entry 4xf4 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure-Based Insights into the Role of the Cys-Tyr Crosslink and Inhibitor Recognition by Mammalian Cysteine Dioxygenase.
Driggers CM, Kean KM, Hirschberger LL, Cooley RB, Stipanuk MH, Karplus PA
J Mol Biol 428 3999-4012 (2016)
PMID: 27477048

Function and Biology Details

Reaction catalysed: 
L-cysteine + O2 = 3-sulfino-L-alanine + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand HCS 1 x HCS
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P43212
Unit cell:
a: 57.6Å b: 57.6Å c: 122.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.191
Expression system: Escherichia coli

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Citations

4 review citations

ROS and RNS signalling: adaptive redox switches through oxidative/nitrosative protein modifications.
Moldogazieva et al. (2018)
3 more

1 mention without citation

Structure-Based Insights into the Role of the Cys-Tyr Crosslink and Inhibitor Recognition by Mammalian Cysteine Dioxygenase.
Driggers et al. (2016)