› 4xga

X-ray diffraction
2.15Å resolution

Crystal structure of BamB and BamA P3-5 complex from E.coli

Released:
DOI: 10.2210/pdb4xga/pdb
PDB entry 4xga coloured by chain, front view.
PDB entry 4xga coloured by chain, side view.
PDB entry 4xga coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA.
Chen Z, Zhan LH, Hou HF, Gao ZQ, Xu JH, Dong C, Dong YH
Acta Crystallogr D Struct Biol 72 236-44 (2016)
PMID: 26894671

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer membrane protein assembly factor BamB Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 39.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77774 (Residues: 20-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamA Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 175-420; Coverage: 31%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains: Surface antigen variable number repeat
Structure domains: membrane protein fhac

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P21212
Unit cell:
a: 109.035Å b: 109.642Å c: 49.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The β-barrel assembly machinery in motion.
Noinaj et al. (2017)
2 more

5 mentions without citation

The big BAM theory: An open and closed case?
Wu et al. (2020)
4 more