› 4xgy

X-ray diffraction
1.49Å resolution

GFP based antibody (fluorobody)

Released:
DOI: 10.2210/pdb4xgy/pdb
PDB entry 4xgy coloured by chain, front view.
PDB entry 4xgy coloured by chain, side view.
PDB entry 4xgy coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.
Chen Y, Huang X, Wang R, Wang S, Shi N
Acta Crystallogr F Struct Biol Commun 71 913-8 (2015)
PMID: 26144238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 33.82 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A059PIQ0 (Residues: 2-173, 174-238; Coverage: 94%)
Gene name: gfp
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand PE5 2 x PE5
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 63.35Å b: 63.35Å c: 125.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Antibody Engineering for Pursuing a Healthier Future.
Saeed et al. (2017)

1 mention without citation

The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.
Chen et al. (2015)