› 4xhi

X-ray diffraction
2.15Å resolution

Crystal structure of native Thosea asigna virus RNA-dependent RNA polymerase (RdRP) at 2.15 Angstrom resolution

Released:
DOI: 10.2210/pdb4xhi/pdb
PDB entry 4xhi coloured by chain, front view.
PDB entry 4xhi coloured by chain, side view.
PDB entry 4xhi coloured by chain, top view.
Source organism: Thosea asigna virus
Primary publication:
The Structure of the RNA-Dependent RNA Polymerase of a Permutotetravirus Suggests a Link between Primer-Dependent and Primer-Independent Polymerases.
Ferrero DS, Buxaderas M, Rodríguez JF, Verdaguer N
PLoS Pathog 11 e1005265 (2015)
PMID: 26625123

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179389 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-dependent RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 705 amino acids
Theoretical weight: 79.54 KDa
Source organism: Thosea asigna virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6A562 (Residues: 1-674; Coverage: 54%)
Gene name: RdRp

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 134.875Å b: 150.825Å c: 100.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.216
Expression system: Trichoplusia ni

Quick links

Citations

4 review citations

A Comprehensive Superposition of Viral Polymerase Structures.
Peersen OB. (2019)
3 more

6 mentions without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
5 more