› 4xkj

X-ray diffraction
3.15Å resolution

a Novel D-lactate Dehydrogenase from Sporolactobacillus sp

Released:
DOI: 10.2210/pdb4xkj/pdb
PDB entry 4xkj coloured by chain, front view.
PDB entry 4xkj coloured by chain, side view.
PDB entry 4xkj coloured by chain, top view.
Source organism: Sporolactobacillus inulinus CASD
Entry authors: Bo Y, Hui D, Xiang L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 36.79 KDa
Source organism: Sporolactobacillus inulinus CASD
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KL04 (Residues: 1-335; Coverage: 100%)
Gene name: SINU_02050
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 61.263Å b: 109.505Å c: 113.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.204 0.292
Expression system: Escherichia coli

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