› 4xm6

X-ray diffraction
2.35Å resolution

Anthrax toxin lethal factor with ligand-induced binding pocket

Released:
DOI: 10.2210/pdb4xm6/pdb
PDB entry 4xm6 coloured by chain, front view.
PDB entry 4xm6 coloured by chain, side view.
PDB entry 4xm6 coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor.
Maize KM, Kurbanov EK, Johnson RL, Amin EA, Finzel BC
FEBS Lett 589 3836-41 (2015)
PMID: 26578066

Function and Biology Details

Reaction catalysed: 
Preferred amino acids around the cleavage site can be denoted BBBBxHx-|-H, in which B denotes Arg or Lys, H denotes a hydrophobic amino acid, and x is any amino acid. The only known protein substrates are mitogen-activated protein (MAP) kinase kinases.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lethal factor Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 60.44 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: P15917 (Residues: 298-809; Coverage: 66%)
Gene names: BXA0172, GBAA_pXO1_0172, lef, pXO1-107
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 41R 1 x 41R
Ligand ZN 1 x ZN
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 57.936Å b: 76.922Å c: 138.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor.
Maize et al. (2015)