› 4xme

X-ray diffraction
1.29Å resolution

Crystal structure of nitrophorin 7 from Rhodnius prolixus at pH 7.8 complexed with NO

Released:
DOI: 10.2210/pdb4xme/pdb
PDB entry 4xme coloured by chain, front view.
PDB entry 4xme coloured by chain, side view.
PDB entry 4xme coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7.
Knipp M, Ogata H, Soavi G, Cerullo G, Allegri A, Abbruzzetti S, Bruno S, Viappiani C, Bidon-Chanal A, Luque FJ
F1000Res 4 45 (2015)
PMID: 26167269

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-7 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.76 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PQK2 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand NO 1 x NO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 38.887Å b: 67.052Å c: 39.082Å
α: 90° β: 117.35° γ: 90°
R-values:
R R work R free
0.172 0.171 0.204
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Elucidation of the heme active site electronic structure affecting the unprecedented nitrite dismutase activity of the ferriheme b proteins, the nitrophorins.
He et al. (2016)
4 more

2 mentions without citation

Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7.
Knipp et al. (2015)
1 more