› 4xml

X-ray diffraction
2.68Å resolution

Crystal structure of Fab of HIV-1 gp120 V3-specific human monoclonal antibody 2424

Released:
DOI: 10.2210/pdb4xml/pdb
PDB entry 4xml coloured by chain, front view.
PDB entry 4xml coloured by chain, side view.
PDB entry 4xml coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
Kumar R, Pan R, Upadhyay C, Mayr L, Cohen S, Wang XH, Balasubramanian P, Nádas A, Seaman MS, Zolla-Pazner S, Gorny MK, Kong XP, Hioe CE
J Virol 89 9090-102 (2015)
PMID: 26109728

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213657 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Light chain of HIV-1 gp120 V3-specific human monoclonal antibody 2424 Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Heavy chain of HIV-1 gp120 V3-specific human monoclonal antibody 2424 Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 69.592Å b: 45.043Å c: 74.778Å
α: 90° β: 96.75° γ: 90°
R-values:
R R work R free
0.204 0.2 0.285
Expression system: Homo sapiens

Quick links

Citations

8 citation in other articles

Structure/Function Studies Involving the V3 Region of the HIV-1 Envelope Delineate Multiple Factors That Affect Neutralization Sensitivity.
Zolla-Pazner et al. (2016)
7 more

1 mention without citation

Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
Kumar et al. (2015)