› 4xn3

X-ray diffraction
1.65Å resolution

Tailspike protein mutant E372A of E. coli bacteriophage HK620 in complex with hexasaccharide

Released:
DOI: 10.2210/pdb4xn3/pdb
PDB entry 4xn3 coloured by chain, front view.
PDB entry 4xn3 coloured by chain, side view.
PDB entry 4xn3 coloured by chain, top view.
Source organism: Enterobacteria phage HK620
Entry authors: Gohlke U, Broeker NK, Heinemann U, Seckler R, Barbirz S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Bacteriophage P22 tailspike N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 596 amino acids
Theoretical weight: 64.19 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AYY6 (Residues: 115-710; Coverage: 84%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG, GLA, GLC, RAM
Carbohydrate polymer
3 bound ligands:
Ligand TRS 1 x TRS
Ligand FMT 2 x FMT
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P321
Unit cell:
a: 74.413Å b: 74.413Å c: 174.612Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.141 0.179
Expression system: Escherichia coli BL21(DE3)

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