› 4xnl

X-ray diffraction
2.9Å resolution

X-ray structure of AlgE2

Released:
DOI: 10.2210/pdb4xnl/pdb
PDB entry 4xnl coloured by chain, front view.
PDB entry 4xnl coloured by chain, side view.
PDB entry 4xnl coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
In meso in situ serial X-ray crystallography of soluble and membrane proteins.
Huang CY, Olieric V, Ma P, Panepucci E, Diederichs K, Wang M, Caffrey M
Acta Crystallogr D Biol Crystallogr 71 1238-56 (2015)
PMID: 26057665

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate production protein AlgE Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 54.48 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P18895 (Residues: 1-490; Coverage: 100%)
Gene names: PA3544, alg76, algE
Sequence domains: Alginate export
Structure domains: Porin

Ligands and Environments

5 bound ligands:
Ligand LDA 8 x LDA
Ligand 78M 4 x 78M
Ligand 78N 1 x 78N
Ligand PE5 1 x PE5
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 47.38Å b: 73.122Å c: 184.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.251 0.287
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Understanding transport by the major facilitator superfamily (MFS): structures pave the way.
Quistgaard et al. (2016)
3 more

2 mentions without citation

In meso in situ serial X-ray crystallography of soluble and membrane proteins.
Huang et al. (2015)
1 more