› 4xnn

X-ray diffraction
1.9Å resolution

Crystal Structure of a GH7 Family Cellobiohydrolase from Daphnia pulex

Released:
DOI: 10.2210/pdb4xnn/pdb
PDB entry 4xnn coloured by chain, front view.
PDB entry 4xnn coloured by chain, side view.
PDB entry 4xnn coloured by chain, top view.
Source organism: Daphnia pulex
Entry authors: Ebrahim A, Hobdey SE, Podkaminer K, Taylor II LE, Beckham GT, Decker SR, Himmel ME, Cragg SM, McGeehan JE

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulose 1,4-beta-cellobiosidase (non-reducing end) Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 48.23 KDa
Source organism: Daphnia pulex
Expression system: Trichoderma reesei QM6a
UniProt:
  • Canonical: E9G5J5 (Residues: 20-464; Coverage: 100%)
Gene names: CEL7A, DAPPUDRAFT_347598
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 128.29Å b: 47.02Å c: 173.5Å
α: 90° β: 108.23° γ: 90°
R-values:
R R work R free
0.166 0.164 0.21
Expression system: Trichoderma reesei QM6a

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