› 4xol

X-ray diffraction
2.91Å resolution

Observing the overall rocking motion of a protein in a crystal - Cubic Ubiquitin crystals.

Released:
DOI: 10.2210/pdb4xol/pdb
PDB entry 4xol coloured by chain, front view.
PDB entry 4xol coloured by chain, side view.
PDB entry 4xol coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Observing the overall rocking motion of a protein in a crystal.
Ma P, Xue Y, Coquelle N, Haller JD, Yuwen T, Ayala I, Mikhailovskii O, Willbold D, Colletier JP, Skrynnikov NR, Schanda P
Nat Commun 6 8361 (2015)
PMID: 26436197

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P4332
Unit cell:
a: 104.946Å b: 104.946Å c: 104.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.269
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Schanda et al. (2016)
5 more

6 mentions without citation

Slow conformational exchange and overall rocking motion in ubiquitin protein crystals.
Kurauskas et al. (2017)
5 more