› 4xou

X-ray diffraction
2.8Å resolution

Crystal structure of the SR Ca2+-ATPase in the Ca2-E1-MgAMPPCP form determined by serial femtosecond crystallography using an X-ray free-electron laser.

Released:
DOI: 10.2210/pdb4xou/pdb
PDB entry 4xou coloured by chain, front view.
PDB entry 4xou coloured by chain, side view.
PDB entry 4xou coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural studies of P-type ATPase-ligand complexes using an X-ray free-electron laser.
Bublitz M, Nass K, Drachmann ND, Markvardsen AJ, Gutmann MJ, Barends TR, Mattle D, Shoeman RL, Doak RB, Boutet S, Messerschmidt M, Seibert MM, Williams GJ, Foucar L, Reinhard L, Sitsel O, Gregersen JL, Clausen JD, Boesen T, Gotfryd K, Wang KT, Olesen C, Møller JV, Nissen P, Schlichting I
IUCrJ 2 409-20 (2015)
PMID: 26175901

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand K 1 x K
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2
Unit cell:
a: 162Å b: 76.3Å c: 151.1Å
α: 90° β: 109° γ: 90°
R-values:
R R work R free
0.305 0.304 0.343

Quick links

Citations

4 review citations

Linking Biochemical and Structural States of SERCA: Achievements, Challenges, and New Opportunities.
Aguayo-Ortiz et al. (2020)
3 more

4 mentions without citation

Serial femtosecond crystallography: A revolution in structural biology.
Martin-Garcia et al. (2016)
3 more