› 4xpm

X-ray diffraction
2.4Å resolution

Crystal structure of EGO-TC

Released:
DOI: 10.2210/pdb4xpm/pdb
PDB entry 4xpm coloured by chain, front view.
PDB entry 4xpm coloured by chain, side view.
PDB entry 4xpm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling.
Powis K, Zhang T, Panchaud N, Wang R, De Virgilio C, Ding J
Cell Res 25 1043-59 (2015)
PMID: 26206314

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-153588 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein MEH1 Chain: A
Molecule details ›
Chain: A
Length: 39 amino acids
Theoretical weight: 4.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02205 (Residues: 146-184; Coverage: 21%)
Gene names: EGO1, GSE2, MEH1, YK106, YKR007W
Protein EGO2 Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3E830 (Residues: 1-75; Coverage: 100%)
Gene names: EGO2, YCR075W-A
Sequence domains: YNR034W-A-like
Structure domains: YNR034W-A-like
Protein SLM4 Chain: C
Molecule details ›
Chain: C
Length: 162 amino acids
Theoretical weight: 18.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38247 (Residues: 1-162; Coverage: 100%)
Gene names: EGO3, GSE1, SLM4, YBR0723, YBR077C
Sequence domains: Protein SLM4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 48.118Å b: 48.118Å c: 223.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.217
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Nutrient sensing and TOR signaling in yeast and mammals.
González et al. (2017)
15 more

4 mentions without citation

Hybrid Structure of the RagA/C-Ragulator mTORC1 Activation Complex.
Su et al. (2017)
3 more