Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Maltose/Cyclodextrin ABC transporter, substrate-binding protein
- p53 tumour suppressor family
- E6 superfamily
- E6 early regulatory protein
- p53, DNA-binding domain
- p53-like transcription factor, DNA-binding domain superfamily
- p53/RUNT-type transcription factor, DNA-binding domain superfamily
- Solute-binding family 1, conserved site
1 more domain
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-136585 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Maltose/maltodextrin-binding periplasmic protein; Ubiquitin-protein ligase E3A
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 41.8 KDa
Source organisms: Expression system: Escherichia coli K-12
UniProt:
Sequence domains: Bacterial extracellular solute-binding protein
Length: 383 amino acids
Theoretical weight: 41.8 KDa
Source organisms: Expression system: Escherichia coli K-12
UniProt:
- Canonical:
P0AEX9 (Residues: 27-392; Coverage: 99%) - Canonical: Q05086 (Residues: 406-417; Coverage: 1%)
Sequence domains: Bacterial extracellular solute-binding protein
Cellular tumor antigen p53
Molecule details ›
Chains: C, D
Length: 199 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
Length: 199 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
- Canonical: P04637 (Residues: 94-292; Coverage: 51%)
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
Protein E6
Molecule details ›
Chains: F, H
Length: 151 amino acids
Theoretical weight: 18.3 KDa
Source organism: Human papillomavirus 16
Expression system: Escherichia coli K-12
UniProt:
Sequence domains: Early Protein (E6)
Structure domains: E6 early regulatory protein
Length: 151 amino acids
Theoretical weight: 18.3 KDa
Source organism: Human papillomavirus 16
Expression system: Escherichia coli K-12
UniProt:
- Canonical: P03126 (Residues: 8-158; Coverage: 96%)
Sequence domains: Early Protein (E6)
Structure domains: E6 early regulatory protein
