› 4xtr

X-ray diffraction
2.05Å resolution

Structure of Get3 bound to the transmembrane domain of Pep12

Released:
DOI: 10.2210/pdb4xtr/pdb
PDB entry 4xtr coloured by chain, front view.
PDB entry 4xtr coloured by chain, side view.
PDB entry 4xtr coloured by chain, top view.
Source organisms:
Primary publication:
Protein targeting. Structure of the Get3 targeting factor in complex with its membrane protein cargo.
Mateja A, Paduch M, Chang HY, Szydlowska A, Kossiakoff AA, Hegde RS, Keenan RJ
Science 347 1152-5 (2015)
PMID: 25745174

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229993 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 39.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Antibody Heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 230 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Antibody Light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 217 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Pep12p Chain: G
Molecule details ›
Chain: G
Length: 37 amino acids
Theoretical weight: 4.58 KDa
Source organism: Saccharomyces cerevisiae Vin13
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand ZN 1 x ZN
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 102.87Å b: 112.03Å c: 153.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structural and Mechanistic Insights into Protein Translocation.
Rapoport et al. (2017)
14 more

11 mentions without citation

Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.
Chio et al. (2017)
10 more