› 4xu4

X-ray diffraction
1.9Å resolution

Crystal structure of a mycobacterial Insig homolog MvINS from Mycobacterium vanbaalenii at 1.9A resolution

Released:
DOI: 10.2210/pdb4xu4/pdb
PDB entry 4xu4 coloured by chain, front view.
PDB entry 4xu4 coloured by chain, side view.
PDB entry 4xu4 coloured by chain, top view.
Source organism: Mycolicibacterium vanbaalenii PYR-1
Primary publication:
PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.
Ren R, Zhou X, He Y, Ke M, Wu J, Liu X, Yan C, Wu Y, Gong X, Lei X, Yan SF, Radhakrishnan A, Yan N
Science 349 187-91 (2015)
PMID: 26160948

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
INSIG protein homolog Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 21.55 KDa
Source organism: Mycolicibacterium vanbaalenii PYR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: A1T557 (Residues: 1-204; Coverage: 100%)
Gene name: Mvan_1475

Ligands and Environments

2 bound ligands:
Ligand BNG 3 x BNG
Ligand DDQ 2 x DDQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R3
Unit cell:
a: 85.498Å b: 85.498Å c: 125.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.203
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Mechanisms and regulation of cholesterol homeostasis.
Luo et al. (2020)
8 more

4 mentions without citation

PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.
Ren et al. (2015)
3 more