› 4xuk

X-ray diffraction
2Å resolution

Crystal structure of hydrolase AbOPH in beta lactamase superfamily

Released:
DOI: 10.2210/pdb4xuk/pdb
PDB entry 4xuk coloured by chain, front view.
PDB entry 4xuk coloured by chain, side view.
PDB entry 4xuk coloured by chain, top view.
Source organism: Acinetobacter sp. NBRC 100985
Primary publication:
Marked enhancement of Acinetobacter sp. organophosphorus hydrolase activity by a single residue substitution Ile211Ala
Chen J, Lu XJ, Chen Q, Pan J, Zhou JH, Xu JH
Bioresour Bioprocess (2015)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 32.28 KDa
Source organism: Acinetobacter sp. NBRC 100985
Expression system: Escherichia coli
UniProt:
  • Canonical: G7GD18 (Residues: 48-338; Coverage: 96%)
Gene name: ACT4_021_01090
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 76.812Å b: 82.735Å c: 99.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.238
Expression system: Escherichia coli

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