› 4xvc

X-ray diffraction
2Å resolution

Crystal structure of an esterase from the bacterial hormone-sensitive lipase (HSL) family

Released:
DOI: 10.2210/pdb4xvc/pdb
PDB entry 4xvc coloured by chain, front view.
PDB entry 4xvc coloured by chain, side view.
PDB entry 4xvc coloured by chain, top view.
Source organism: environmental samples
Primary publication:
Interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family.
Li PY, Chen XL, Ji P, Li CY, Wang P, Zhang Y, Xie BB, Qin QL, Su HN, Zhou BC, Zhang YZ, Zhang XY
J Biol Chem 290 11188-98 (2015)
PMID: 25771540

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase E40 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 317 amino acids
Theoretical weight: 34.3 KDa
Source organism: environmental samples
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand PMS 8 x PMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 74.63Å b: 76.172Å c: 130.186Å
α: 74.04° β: 75.15° γ: 72.6°
R-values:
R R work R free
0.198 0.196 0.232
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Microbial esterases and ester prodrugs: An unlikely marriage for combating antibiotic resistance.
Larsen et al. (2019)
22 other articles

10 mentions without citation

Interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family.
Li et al. (2015)
9 more