› 4xw3

X-ray diffraction
2Å resolution

Crystal structure of the SPRY domain of the human DEAD-box protein DDX1

Released:
DOI: 10.2210/pdb4xw3/pdb
PDB entry 4xw3 coloured by chain, front view.
PDB entry 4xw3 coloured by chain, side view.
PDB entry 4xw3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the SPRY domain of the human RNA helicase DDX1, a putative interaction platform within a DEAD-box protein.
Kellner JN, Meinhart A
Acta Crystallogr F Struct Biol Commun 71 1176-88 (2015)
PMID: 26323305

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92499 (Residues: 72-283; Coverage: 29%)
Gene name: DDX1
Sequence domains: SPRY domain
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 45.06Å b: 76.14Å c: 122.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

DEAD-box helicases: the Yin and Yang roles in viral infections.
Meier-Stephenson et al. (2018)
4 other articles

3 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
2 more