› 4xwn

X-ray diffraction
2.88Å resolution

Complex structure of catalytic domain of Clostridium Cellulovorans Exgs and Cellotetraose

Released:
DOI: 10.2210/pdb4xwn/pdb
PDB entry 4xwn coloured by chain, front view.
PDB entry 4xwn coloured by chain, side view.
PDB entry 4xwn coloured by chain, top view.
Source organism: Clostridium cellulovorans
Primary publication:
Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage.
Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, Liang PH, Liaw YC
Acta Crystallogr F Struct Biol Commun 71 1264-72 (2015)
PMID: 26457517

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Dockerin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 681 amino acids
Theoretical weight: 75.89 KDa
Source organism: Clostridium cellulovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: O65986 (Residues: 32-674; Coverage: 91%)
Gene name: exgS
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1, NSRRC BEAMLINE BL13C1
Spacegroup: P41212
Unit cell:
a: 108.856Å b: 108.856Å c: 182.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.193
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Genomically Defined Paenibacillus polymyxa ND24 for Efficient Cellulase Production Utilizing Sugarcane Bagasse as a Substrate.
Bohra et al. (2019)

1 mention without citation

Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage.
Tsai et al. (2015)