› 4xxf

X-ray diffraction
1.34Å resolution

L-fuculose 1-phosphate aldolase from Glaciozyma antarctica PI12

Released:
DOI: 10.2210/pdb4xxf/pdb
PDB entry 4xxf coloured by chain, front view.
PDB entry 4xxf coloured by chain, side view.
PDB entry 4xxf coloured by chain, top view.
Source organism: Glaciozyma antarctica
Primary publication:
Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12.
Jaafar NR, Littler D, Beddoe T, Rossjohn J, Illias RM, Mahadi NM, Mackeen MM, Murad AM, Abu Bakar FD
Acta Crystallogr F Struct Biol Commun 72 831-839 (2016)
PMID: 27827354

Function and Biology Details

Reaction catalysed: 
L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class II aldolase/adducin N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.17 KDa
Source organism: Glaciozyma antarctica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0J9X279 (Residues: 1-258; Coverage: 100%)
Sequence domains: Class II Aldolase and Adducin N-terminal domain
Structure domains: Class II aldolase/adducin N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P4212
Unit cell:
a: 84.33Å b: 84.33Å c: 78.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12.
Yusof et al. (2021)
1 more

1 mention without citation

Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12.
Jaafar et al. (2016)