› 4xyz

X-ray diffraction
1.65Å resolution

Crystal structure of K33 linked di-Ubiquitin

Released:
DOI: 10.2210/pdb4xyz/pdb
PDB entry 4xyz coloured by chain, front view.
PDB entry 4xyz coloured by chain, side view.
PDB entry 4xyz coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Assembly and structure of Lys33-linked polyubiquitin reveals distinct conformations.
Kristariyanto YA, Choi SY, Rehman SA, Ritorto MS, Campbell DG, Morrice NA, Toth R, Kulathu Y
Biochem J 467 345-52 (2015)
PMID: 25723849

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P0CH28 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

4 bound ligands:
Ligand EDO 1 x EDO
Ligand IOD 1 x IOD
Ligand ACT 1 x ACT
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 29.48Å b: 57.017Å c: 33.984Å
α: 90° β: 95.45° γ: 90°
R-values:
R R work R free
0.165 0.162 0.214

Quick links

Citations

14 review citations

Ubiquitin modifications.
Swatek et al. (2016)
13 more

7 mentions without citation

Bacteria make surgical strikes on host ubiquitin signaling.
Franklin et al. (2021)
6 more