› 4y00

X-ray diffraction
3Å resolution

Crystal Structure of Human TDP-43 RRM1 Domain with D169G Mutation in Complex with an Unmodified Single-stranded DNA

Released:
DOI: 10.2210/pdb4y00/pdb
PDB entry 4y00 coloured by chain, front view.
PDB entry 4y00 coloured by chain, side view.
PDB entry 4y00 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of disease-related TDP-43 D169G mutation: linking enhanced stability and caspase cleavage efficiency to protein accumulation.
Chiang CH, Grauffel C, Wu LS, Kuo PH, Doudeva LG, Lim C, Shen CK, Yuan HS
Sci Rep 6 21581 (2016)
PMID: 26883171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118299 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TAR DNA-binding protein 43 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 103 amino acids
Theoretical weight: 12.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13148 (Residues: 101-191; Coverage: 22%)
Gene names: TARDBP, TDP43
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (5'-D(P*TP*TP*GP*AP*GP*CP*GP*T)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P3221
Unit cell:
a: 97.636Å b: 97.636Å c: 96.871Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.275 0.266 0.295
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Molecular Mechanisms of TDP-43 Misfolding and Pathology in Amyotrophic Lateral Sclerosis.
Prasad et al. (2019)
9 more

3 mentions without citation

Structural Insights Into TDP-43 and Effects of Post-translational Modifications.
François-Moutal et al. (2019)
2 more