› 4y0w

X-ray diffraction
2.5Å resolution

YeaZ from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4y0w/pdb
PDB entry 4y0w coloured by chain, front view.
PDB entry 4y0w coloured by chain, side view.
PDB entry 4y0w coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO579
Primary publication:
Crystal structure of YeaZ from Pseudomonas aeruginosa.
Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G
Biochem Biophys Res Commun 470 460-465 (2016)
PMID: 26768361

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 232 amino acids
Theoretical weight: 25.03 KDa
Source organism: Pseudomonas aeruginosa PAO579
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140UHA7 (Residues: 1-226; Coverage: 100%)
Gene name: A161_18155
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

1 bound ligand:
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 78.03Å b: 198.443Å c: 161.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Conservation and Diversification of tRNA t6A-Modifying Enzymes across the Three Domains of Life.
Su et al. (2022)
2 more

2 mentions without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)
1 more