› 4y25

X-ray diffraction
2.82Å resolution

Bacterial polysaccharide outer membrane secretin

Released:
DOI: 10.2210/pdb4y25/pdb
PDB entry 4y25 coloured by chain, front view.
PDB entry 4y25 coloured by chain, side view.
PDB entry 4y25 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Basis for Translocation of a Biofilm-supporting Exopolysaccharide across the Bacterial Outer Membrane.
Wang Y, Andole Pannuri A, Ni D, Zhou H, Cao X, Lu X, Romeo T, Huang Y
J Biol Chem 291 10046-57 (2016)
PMID: 26957546

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly-beta-1,6-N-acetyl-D-glucosamine export protein Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 36.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69434 (Residues: 511-807; Coverage: 38%)
Gene names: JW1010, b1024, pgaA, ycdS
Sequence domains: PgaA membrane beta barrel domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6322
Unit cell:
a: 143.972Å b: 143.972Å c: 90.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.232 0.283
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.
Horne et al. (2020)
4 more

11 mentions without citation

Structural Basis for Translocation of a Biofilm-supporting Exopolysaccharide across the Bacterial Outer Membrane.
Wang et al. (2016)
10 more