› 4y30

X-ray diffraction
2.1Å resolution

Crystal structure of human protein arginine methyltransferase PRMT6 bound to SAH and EPZ020411

Released:
DOI: 10.2210/pdb4y30/pdb
PDB entry 4y30 coloured by chain, front view.
PDB entry 4y30 coloured by chain, side view.
PDB entry 4y30 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Aryl Pyrazoles as Potent Inhibitors of Arginine Methyltransferases: Identification of the First PRMT6 Tool Compound.
Mitchell LH, Drew AE, Ribich SA, Rioux N, Swinger KK, Jacques SL, Lingaraj T, Boriack-Sjodin PA, Waters NJ, Wigle TJ, Moradei O, Jin L, Riera T, Porter-Scott M, Moyer MP, Smith JJ, Chesworth R, Copeland RA
ACS Med Chem Lett 6 655-9 (2015)
PMID: 26101569

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein arginine N-methyltransferase 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96LA8 (Residues: 25-375; Coverage: 94%)
Gene names: HRMT1L6, PRMT6
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SAH 2 x SAH
Ligand 49L 2 x 49L
Ligand MG 1 x MG
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 99.672Å b: 99.672Å c: 89.252Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.221
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Protein arginine methylation: from enigmatic functions to therapeutic targeting.
Wu et al. (2021)
26 more

8 mentions without citation

Inhibitors of Protein Methyltransferases and Demethylases.
Kaniskan et al. (2018)
7 more