› 4y3i

X-ray diffraction
1.69Å resolution

PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, low dose

Released:
DOI: 10.2210/pdb4y3i/pdb
PDB entry 4y3i coloured by chain, front view.
PDB entry 4y3i coloured by chain, side view.
PDB entry 4y3i coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. radiodurans phytochrome.
Li F, Burgie ES, Yu T, Héroux A, Schatz GC, Vierstra RD, Orville AM
J Am Chem Soc 137 2792-5 (2015)
PMID: 25650486

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 34.46 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 4-321; Coverage: 42%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 1 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 89.481Å b: 51.917Å c: 80.339Å
α: 90° β: 116.22° γ: 90°
R-values:
R R work R free
0.192 0.19 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

9 citation in other articles

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.
Roessler et al. (2016)
8 more

2 mentions without citation

To Tie or Not to Tie? That Is the Question.
Dabrowski-Tumanski et al. (2017)
1 more