› 4y4n

X-ray diffraction
2.1Å resolution

Thiazole synthase Thi4 from Methanococcus igneus

Released:
DOI: 10.2210/pdb4y4n/pdb
PDB entry 4y4n coloured by chain, front view.
PDB entry 4y4n coloured by chain, side view.
PDB entry 4y4n coloured by chain, top view.
Source organism: Methanotorris igneus Kol 5
Primary publication:
Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.
Zhang X, Eser BE, Chanani PK, Begley TP, Ealick SE
Biochemistry 55 1826-38 (2016)
PMID: 26919468

Function and Biology Details

Reaction catalysed: 
NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine thiazole synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 286 amino acids
Theoretical weight: 30.84 KDa
Source organism: Methanotorris igneus Kol 5
Expression system: Escherichia coli
UniProt:
  • Canonical: F6BCS4 (Residues: 1-263; Coverage: 100%)
Gene names: Metig_0735, thi4
Sequence domains: Thi4 family
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
Ligand FE2 8 x FE2
Ligand 48H 8 x 48H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P31
Unit cell:
a: 101.302Å b: 101.302Å c: 204.309Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.199 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Vitamin Biosynthesis as an Antifungal Target.
Meir et al. (2018)
1 more

2 mentions without citation

Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases.
Zhang et al. (2016)
1 more