› 4y4q

X-ray diffraction
2.16Å resolution

Crystal structure of sortase B from Type II pilus of Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb4y4q/pdb
PDB entry 4y4q coloured by chain, front view.
PDB entry 4y4q coloured by chain, side view.
PDB entry 4y4q coloured by chain, top view.
Source organism: Streptococcus pneumoniae PCS8235
Primary publication:
A structural snapshot of type II pilus formation in Streptococcus pneumoniae.
Shaik MM, Lombardi C, Maragno Trindade D, Fenel D, Schoehn G, Di Guilmi AM, Dessen A
J Biol Chem 290 22581-92 (2015)
PMID: 26198632

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sortase, SrtB family Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.51 KDa
Source organism: Streptococcus pneumoniae PCS8235
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL14 (Residues: 29-243; Coverage: 89%)
Gene name: PCS8235_00725
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 74.15Å b: 101.14Å c: 143.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
2 more

2 mentions without citation

Pilus biogenesis of Gram-positive bacteria: Roles of sortases and implications for assembly.
Khare et al. (2017)
1 more