› 4y7i

X-ray diffraction
2.8Å resolution

Crystal Structure of MTMR8

Released:
DOI: 10.2210/pdb4y7i/pdb
PDB entry 4y7i coloured by chain, front view.
PDB entry 4y7i coloured by chain, side view.
PDB entry 4y7i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the catalytic phosphatase domain of MTMR8: implications for dimerization, membrane association and reversible oxidation.
Yoo KY, Son JY, Lee JU, Shin W, Im DW, Kim SJ, Ryu SE, Heo YS
Acta Crystallogr D Biol Crystallogr 71 1528-39 (2015)
PMID: 26143924

Function and Biology Details

Reactions catalysed: 
1-phosphatidyl-1D-myo-inositol 3-phosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol + phosphate
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myotubularin-related protein 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 45.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96EF0 (Residues: 122-505; Coverage: 55%)
Gene name: MTMR8
Sequence domains: Myotubularin-like phosphatase domain

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 69.573Å b: 77.188Å c: 197.793Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.247
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Genetic Background of Abnormalities in Metabolic Pathways of Phosphoinositides and Their Linkage with the Myotubular Myopathies, Neurodegenerative Disorders, and Carcinogenesis.
Derkaczew et al. (2023)
2 other articles

1 mention without citation

Crystal Structure of Human Myotubularin-Related Protein 1 Provides Insight into the Structural Basis of Substrate Specificity.
Bong et al. (2016)