› 4y7l

X-ray diffraction
1.51Å resolution

T6SS protein TssM C-terminal domain (869-1107) from EAEC

Released:
DOI: 10.2210/pdb4y7l/pdb
PDB entry 4y7l coloured by chain, front view.
PDB entry 4y7l coloured by chain, side view.
PDB entry 4y7l coloured by chain, top view.
Source organism: Escherichia coli 2-156-04_S3_C3
Primary publication:
Biogenesis and structure of a type VI secretion membrane core complex.
Durand E, Nguyen VS, Zoued A, Logger L, Péhau-Arnaudet G, Aschtgen MS, Spinelli S, Desmyter A, Bardiaux B, Dujeancourt A, Roussel A, Cambillau C, Cascales E, Fronzes R
Nature 523 555-60 (2015)
PMID: 26200339

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type VI secretion protein IcmF Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 26.51 KDa
Source organism: Escherichia coli 2-156-04_S3_C3
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0M3KL15 (Residues: 1-240; Coverage: 100%)
Gene name: AC43_2230
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 64.03Å b: 64.03Å c: 249.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.203
Expression system: Escherichia coli BL21

Quick links

Citations

32 review citations

Aim, Load, Fire: The Type VI Secretion System, a Bacterial Nanoweapon.
Cianfanelli et al. (2016)
31 more

1 mention without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)