› 4y7m

X-ray diffraction
1.92Å resolution

T6SS protein TssM C-terminal domain (835-1129) from EAEC

Released:
DOI: 10.2210/pdb4y7m/pdb
PDB entry 4y7m coloured by chain, front view.
PDB entry 4y7m coloured by chain, side view.
PDB entry 4y7m coloured by chain, top view.
Source organisms:
Primary publication:
Biogenesis and structure of a type VI secretion membrane core complex.
Durand E, Nguyen VS, Zoued A, Logger L, Péhau-Arnaudet G, Aschtgen MS, Spinelli S, Desmyter A, Bardiaux B, Dujeancourt A, Roussel A, Cambillau C, Cascales E, Fronzes R
Nature 523 555-60 (2015)
PMID: 26200339

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207323 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hi113 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 13.9 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Type VI secretion protein IcmF Chains: C, D
Molecule details ›
Chains: C, D
Length: 295 amino acids
Theoretical weight: 32.43 KDa
Source organism: Escherichia coli 2-156-04_S3_C3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL16 (Residues: 1-295; Coverage: 100%)
Gene name: AC43_2230
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P64
Unit cell:
a: 95.23Å b: 95.23Å c: 172.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

Aim, Load, Fire: The Type VI Secretion System, a Bacterial Nanoweapon.
Cianfanelli et al. (2016)
31 more

2 mentions without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)
1 more