› 4y90

X-ray diffraction
2.1Å resolution

Crystal structure of Triosephosphate Isomerase from Deinococcus radiodurans

Released:
DOI: 10.2210/pdb4y90/pdb
Model geometry
Fit model/data
PDB entry 4y90 coloured by chain, front view.
PDB entry 4y90 coloured by chain, side view.
PDB entry 4y90 coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Reversibility and two state behaviour in the thermal unfolding of oligomeric TIM barrel proteins.
Romero-Romero S, Costas M, Rodríguez-Romero A, Alejandro Fernández-Velasco D
Phys Chem Chem Phys 17 20699-714 (2015)
PMID: 26206330

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 25.62 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RUP5 (Residues: 1-244; Coverage: 100%)
Gene names: DR_1339, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 9 x GOL
Ligand CA 5 x CA
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R32
Unit cell:
a: 169.62Å b: 169.62Å c: 202.266Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.136 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

De novo design of a four-fold symmetric TIM-barrel protein with atomic-level accuracy.
Huang et al. (2016)
8 more

1 mention without citation

On a Robust, Sensitive Cell-Free Method for Pseudomonas Sensing and Quantification in Microfluidic Templated Hydrogels.
Seto J. (2019)