› 4y96

X-ray diffraction
1.58Å resolution

Crystal structure of Triosephosphate Isomerase from Gemmata obscuriglobus

Released:
DOI: 10.2210/pdb4y96/pdb
PDB entry 4y96 coloured by chain, front view.
PDB entry 4y96 coloured by chain, side view.
PDB entry 4y96 coloured by chain, top view.
Source organism: Gemmata obscuriglobus
Primary publication:
Reversibility and two state behaviour in the thermal unfolding of oligomeric TIM barrel proteins.
Romero-Romero S, Costas M, Rodríguez-Romero A, Alejandro Fernández-Velasco D
Phys Chem Chem Phys 17 20699-714 (2015)
PMID: 26206330

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.06 KDa
Source organism: Gemmata obscuriglobus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL18 (Residues: 1-255; Coverage: 100%)
Gene names: tim, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand PO4 2 x PO4
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 124.778Å b: 124.778Å c: 134.231Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

De novo design of a four-fold symmetric TIM-barrel protein with atomic-level accuracy.
Huang et al. (2016)
8 more