› 4yal

X-ray diffraction
1.88Å resolution

Reduced CYPOR with 2'-AMP

Released:
DOI: 10.2210/pdb4yal/pdb
PDB entry 4yal coloured by chain, front view.
PDB entry 4yal coloured by chain, side view.
PDB entry 4yal coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone.
Rwere F, Xia C, Im S, Haque MM, Stuehr DJ, Waskell L, Kim JJ
J Biol Chem 291 14639-61 (2016)
PMID: 27189945

Function and Biology Details

Reaction catalysed: 
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH--cytochrome P450 reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 622 amino acids
Theoretical weight: 70.68 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00388 (Residues: 57-678; Coverage: 92%)
Gene name: Por
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN

Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand 2AM 2 x 2AM
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 101.907Å b: 115.418Å c: 118.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.228
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Roles of Ferredoxin-NADP+ Oxidoreductase and Flavodoxin in NAD(P)H-Dependent Electron Transfer Systems.
Iyanagi T. (2022)
11 other articles

3 mentions without citation

Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone.
Rwere et al. (2016)
2 more