› 4yed

X-ray diffraction
1.9Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA threonylcarbamoyladenosine dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 271 amino acids
Theoretical weight: 29.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46927 (Residues: 1-268; Coverage: 100%)
Gene names: JW2783, b2812, csdL, tcdA, ygdL
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand AMP 4 x AMP
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 65.555Å b: 97.117Å c: 83.337Å
α: 90° β: 111.65° γ: 90°
R-values:
R R work R free
0.161 0.159 0.2
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Diverse Mechanisms of Sulfur Decoration in Bacterial tRNA and Their Cellular Functions.
Zheng et al. (2017)
3 other articles