4yg1

X-ray diffraction
3.25Å resolution

HipB-O1-O2 complex/P21212 crystal form

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118315 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Antitoxin HipB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 72 amino acids
Theoretical weight: 8.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23873 (Residues: 1-72; Coverage: 82%)
Gene names: JW1501, b1508, hipB
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (48-MER) Chain: F
Molecule details ›
Chain: F
Length: 48 nucleotides
Theoretical weight: 14.86 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (48-MER) Chain: T
Molecule details ›
Chain: T
Length: 48 nucleotides
Theoretical weight: 14.7 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 293.887Å b: 54.46Å c: 47.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.287
Expression systems:
  • Escherichia coli
  • Not provided