› 4yhc

X-ray diffraction
2.05Å resolution

Crystal structure of the WD40 domain of SCAP from fission yeast

Released:
DOI: 10.2210/pdb4yhc/pdb
Model geometry
Fit model/data
PDB entry 4yhc coloured by chain, front view.
PDB entry 4yhc coloured by chain, side view.
PDB entry 4yhc coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structure of the WD40 domain of SCAP from fission yeast reveals the molecular basis for SREBP recognition.
Gong X, Li J, Shao W, Wu J, Qian H, Ren R, Espenshade P, Yan N
OpenAccess logo Cell Res 25 401-11 (2015)
PMID: 25771684

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128823 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sterol regulatory element-binding protein cleavage-activating protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 52.79 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O43043 (Residues: 567-961, 986-1054; Coverage: 43%)
Gene names: SPBC3B9.15c, scp1

Ligands and Environments

1 bound ligand:
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 38.009Å b: 109.714Å c: 103.454Å
α: 90° β: 100.46° γ: 90°
R-values:
R R work R free
0.18 0.177 0.229
Expression system: Escherichia coli

Quick links

Citations

5 review citations

SREBP-regulated lipid metabolism: convergent physiology - divergent pathophysiology.
Shimano et al. (2017)
4 more

2 mentions without citation

Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization.
Gong et al. (2016)
1 more